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B., Lindenau, J., Seyfried, J., et al
Thus, roGFP2 oxidation does not efficiently occur via a Grx(SSR) intermediate and we can exclude the dithiol mechanism and monothiol mechanism (i) for roGFP2 oxidation (reverse reactions in Fig
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The GSSG-dependent oxidation of roGFP2 by fused PfGrx variants is two to three orders of magnitude faster than the oxidation by unfused PfGrx(SSG) variants and more than seven orders of magnitude faster than the uncatalyzed reaction with GSSG (Table 1)
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